First Response® Malaria Ag. pLDH/HRP2 Combo Card Test is intended to be performed by trained users (In either laboratory or point of care settings) as qualitative screening test for detection of P. falciparum, P. vivax, P. ovale and P. malariae. The test is intended for use with whole blood specimens (capillary or venous blood). Venous blood with the anti-coagulants heparin, EDTA or citrate do not affect the test results. This kit is not intended to be used for screening of blood donors. First Response® Malaria Ag. pLDH/HRP2 Combo Card Test is based on principle of immunochromatography in which nitrocellulose membrane is pre-coated with two monoclonal antibodies as two separate lines. One monoclonal antibody (test line PAN) is PAN specific to lactate dehydrogenase (pLDH) of the Plasmodium species (Plasmodium falciparum, P. vivax, P. ovale and P. malariae.) and the other line (test line P.f.) consists of a monoclonal antibody specific to Histidine Rich Protein 2 (HRP2) of the Plasmodium falciparum. When the test sample along with assay Buffer flows through the nitrocellulose membrane, monoclonal antibodies conjugated with colloidal gold, which are PAN specific to pLDH and P. falciparum specific to HRP2 binds to Plasmodium antigens released from the lysed blood sample. – Read IFU for more information.